Nuclear Matrix Attachment Regions and Transgene Expression in Plants
نویسندگان
چکیده
منابع مشابه
Nuclear Matrix Attachment Regions and Transgene Expression in Plants.
DNA sequences called matrix attachment regions (MARs) or scaffold attachment regions (SARs) have recently attracted much attention because of their perceived capacity to increase levels of transgene expression and reduce transformant-to-transformant variation of transgene expression in both plants and animals. Work with these sequences is in its early stages and data that seem to be contradicto...
متن کاملEffect of nuclear matrix attachment regions on transgene expression in tobacco plants.
Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on r...
متن کاملThe influences of two plant nuclear matrix attachment regions (MARs) on gene expression in transgenic plants.
Nuclear matrix attachment regions (MARs) are thought to influence gene expression by anchoring active chromatin to the proteinaceous nuclear matrix. In this study, two plant DNA fragments with strong MAR activity were selected and tested for their effects on expression of a linked reporter gene in transgenic tobacco. One MAR was isolated from the 5' flanking region of a pea vicilin gene previou...
متن کاملMatrix attachment regions and regulated transcription increase and stabilize transgene expression.
Transgene silencing has been shown to be associated with strong promoters, but it is not known whether the propensity for silencing is caused by the level of transcription, or some other property of the promoter. If transcriptional activity fosters silencing, then transgenes with inducible promoters may be less susceptible to silencing. To test this idea, a doxycycline-inducible luciferase tran...
متن کاملHuman matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.
Germ line transformation of white- Drosophila embryos with P-element vectors containing white expression cassettes results in flies with different eye color phenotypes due to position effects at the sites of transgene insertion. These position effects can be cured by specific DNA elements, such as the Drosophila scs and scs' elements, that have insulator activity in vivo. We have used this syst...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1996
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.110.1.15